The use of motility as a measure of sperm quality in fish is reviewed. Computer-assisted sperm analysis (CASA) provides a simple and rapid quantitative assessment of the quality of fish sperm and may predict its ability to fertilize eggs. It has been used to monitor the effects of heavy metal pollutants, such as mercury and tributyltin, on sperm quality; to select broodstock; to improve the efficiency of cryopreservation and storage, and to optimize conditions for fertilization. In combination with CASA, morphological measurements can be used to determine the causes of reduced sperm motility. Technical details for the use of CASA are described.
Almost all manual semen analysis methods lack accuracy and reproducibility and with poor standardization across the globe do not give confidence to users of any laboratory service whether they are patients or clinicians. Even those recommended by the World Health Organisation (WHO, 2010) are prone to error with especially motility being nothing more than an ‘educated guess’. The WHO (2010) continue to recommend the haemocytometer as a method of choice and whilst the haemocytometer is not only relatively inexpensive and reliable, like any method it is not without limitations and associated error including Differences in manufacturing standards, age and quality of chambers, application of the cover glass, to dilution and mathematics. All of these contribute to the ‘often’ poor results shown in external quality assurance (EQA) scheme where often wide-ranging sperm counts can be given for the same sample (see below)
EQA scheme in the UK showing a wide range of sperm counts for the same sample
In the absence of any other recommendation, a good quality CASA Semen Analysis System should be demonstrated as giving results which equate to the haemocytometer. Up to now, famous CASA Sperm Analysis System has SCA System, Microptic system, Proiser system etc.
And for CASA Semen Analysis, disposable Sperm Counting Slide must require. Many different slide brank in the world, famous as Leja slide, Cell-vision slide, Cell-VU Slide, Markle slide, ASCEN Slide from China.
With its first-class independent R & D capability, the company aims to be the first-class brand in the field of CASA sperm analysis and inspection,become a professional independent R&D, manufacturing and sales manufacturer of medical products such as sperm analysis instruments and sperm counting slide and so on.
Our ASCEN CASA disposable counting chamber slide is perfectly match with the world popular CASA Software system for automatic semen analysis.
1. Hamilton Thorne Computer Assisted Sperm Analysis
CASA (Computer Assisted Sperm Analysis) image analysis systems are designed to bring quality, efficiency, and reliability to studies of reproductive cells in the human fertility*, animal sciences, and reproductive toxicology fields. Our sperm analyzers assist researchers and clinicians in analyzing sperm motility, morphology, or other characteristics in human fertility, toxicology, research, and animal applications. IVOS II CASA Sperm Analyzer, CEROS II Microscope Sperm Analyzer, TOX IVOS II CASA Semen Analyzer
2. MICROPTIC CASA Systems SCA CASA System for semen analysis allows the accurate, repetitive and automatic assessment of the following sperm parameters: motility, concentration, morphology, DNA fragmentation, vitality, acrosome reaction and leukocytes.
3. Minitube AndroVision: CASA software with PC and monitor
AndroVision CASA Analyzer is a highly precise automated system for computerized semen analysis. It not only provides classical analyses of motility, concentration and morphology, but also various fluorescence based assessments of sperm functionality. The basic system for the analysis of motility and concentration is complemented by optional software modules.
4. computer assisted semen analysis System - GenePro
All these CASA Computer Assisted Semen Analysis Software System Analyzer popular in the market
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