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Why China ASCEN CASA Sperm Counting Chamber Slide can replace IMV LEJA Chamber Slide for IVOSII IVOS PRO CASA Sperm Analyzer?

Why China ASCEN CASA Sperm Counting Chamber Slide can replace IMV LEJA Chamber Slide for IVOSII IVOS PRO CASA Sperm Analyzer?


As we all know that the world famous casa sperm semen analysis software system IMV IVOSII and IVOS PRO CASA Sperm Analyzer,but you mainly know that they must required IMV LEJA Chamber Slide for the casa software system analysis sperm quality,do you know which China brand CASA Sperm Chamber Slide can replace IMV LEJA Chamber Slide and perfect match IMV IVOSII and IVOS PRO CASA Sperm Analysis Software System?


In China,China ASCEN brand CASA Sperm Counting Chamber Slide is the most famous and perfect match with IMV IVOSII and IVOS PRO CASA Sperm Analyzer,so Why China ASCEN CASA Sperm Counting Chamber Slide can be alternative to IMV LEJA Chamber Slide?


China ASCEN Sperm Chamber Slides offer greater precision and reliability than other commercially available slides, This is why breeding centers, veterinary institutions, and research facilities choose China ASCEN Sperm Chamber Slide replace IMV LEJA Chamber Slide.


High Technology and PrecisionAccuracy in semen analysis is critical, as it directly impacts the semen dilution ratio calculated by CASA systems, thereby affecting economic outcomes.


The precision of calibrated slides correlates with consistent chamber height. ASCEN Sperm Chamber Slide has developed a production technique that detects and verifies height constancy.


To achieve this, ASCEN implements rigorous quality control:

● Raw material control and traceability

● Toxicity control

● Chamber height control

● 18-point visual inspection...


High-Tech MaterialsThe premium quality of the glass ensures precise analysis of semen quality by CASA systems. Semen analysis in dust-free chambers prevents dust accumulation on slides and the risk of erroneous readings, enhancing the safety of the analytical process.


All components of ASCEN Sperm Chamber Slides are sperm-safe. Each batch undergoes sperm toxicity control and is accompanied by a test certificate.Chamber Filling Control Chamber filling is one of the most critical steps for reliable counting and controlling semen flow. To ensure perfect reproducibility, also ASCEN CASA Sperm Chamber Slide employs a unique coating that optimizes chamber filling through capillary action. Sperm do not adhere to the slide, distribute evenly, and no bubbles form.


So how to use China ASCEN CASA Sperm Chamber Slide correctly when you are using it to match with CASA Software System analysis sperm quality?



Principle :
ASCEN CASA slides are suitable for the analysis of sperm motility and concentration
with the advanced CASA systems. However, can also be used for manual assessments
in case a calibrated eye-piece reticule is mounted in the microscope ocular.

Sperm cells distribute homogenously over the chamber by capillary action. As
disposable chambers, no washing is required after use. This fact minimizes the risk of

Product characteristics
1 Box content: 25 slides/100 tests

Material required :
Warming stage (In case of assessing motility at 37ºC)
Pipette and tips
A piece of paper tissue

Storage and stability :
ASCEN CASA Counting chambers should be stored in a closed box, protected from
sunlight, and at room temperature. The shelf life of the slides is two years, but a haze
can form inside the chamber. This can be washed away after filling without affecting
the functioning of the chamber.

Step 1: Aspirate ~ 2,5 -4.5μl of fully liquefied and homogenized semen.

Carefully place the tip of the pipette on the slide within the boundaries of the
loading area. Be careful not to touch the coverslip to prevent premature filling of the
chamber.

In just one step, push the piston down till the first resistance to load the loading area.
The chamber will be filled by capillarity. If chamber is loaded in more than one step
air-bubbles could be inserted inside the chamber. Thus, affecting the reliability of
results.
Depending on the viscosity of the sample filling will take 3 - 20 seconds or even more.

Step 2: Carefully, take away excess of sample with a paper towel or similar to stop the

drifting of cells inside the chamber


For performing automated semen assessment with the advanced CASA systems like
IVOSII Androscope,IVOS PRO etc.., start the analysis immediately after Step 2 to
prevent evaporation of the sample.

Manual assessments should be performed according to WHO standards and with the
help of a calibrated eye-piece reticule on the microscope ocular, considering the
magnification factor used on each case

If the analysis of sample concentration cannot be performed directly after filling, and
if no motility analysis is needed, the inlet and air outlet of the chamber can be sealed
with nail polish to prevent evaporation.

It’s recommended to prewarm slides and pipette tips at 37ºC to prevent temperature
shock during sample handling.


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